Frazionamento dei peptidi

Miglioramento dell’identificazione delle proteine mediante LC-MS/MS in campioni biologici complessi come il plasma, grazie al frazionamento dei peptidi a pH basico.

02 sf peptide fractionation v2

BioSPE™ PepFrac è utilizzato per il frazionamento dei peptidi in base all’idrofobicità a pH basico. Essendo questo metodo di frazionamento ortogonale alla separazione dei peptidi mediante cromatografia liquida in fase inversa, la complessità del campione è ridotta per il sequenziamento profondo del proteoma e l’analisi quantitativa.

Il kit BioSPE™ PepFrac è composto da:

  • Colonne di frazionamento basate su un’innovativa resina a fase inversa
  • Adattatori (per la centrifugazione dei puntali) e/o strumenti di raccolta (provette da centrifuga o piastre di raccolta). Ogni kit contiene nove strumenti di raccolta (uno per le fasi di condizionamento/caricamento/lavaggio e uno per ogni frazione di eluizione)
  • Un protocollo dettagliato per il frazionamento rapido ed efficiente dei peptidi (fino a 8 frazioni)
  • Soluzione di trietilammina (TEA)

Per determinare il formato e la capacità più adatti alle proprie esigenze, consultare le tabelle nella scheda “Formati e capacità”.

ELENCO PRODOTTI

Nome del prodotto Prezzo del prodotto Product SKU Azioni del carrello
kit 96 plate microelution biospe pepfrac 600x600

BioSPE™ PepFrac – 96 wellplate for microelution & collection plates

96 Wellplate for peptide fractionation at basic pH - Tip-shaped wells - High Capacity - 1/pk + Collection plate - 1mL - 9/pk + Triethylamine 0.1% (200mL)

Ref. : KT-Coll-µ96W-PepFrac.H8.1
kit 96 plate microelution biospe pepfrac 600x600

BioSPE™ PepFrac – 96 wellplate for microelution & collection plates

96 Wellplate for peptide fractionation at basic pH - Tip-shaped wells - Standard Capacity - 1/pk + Collection plate - 1mL - 9/pk + Triethylamine 0.1% (200mL)

Ref. : KT-Coll-µ96W-PepFrac.S8.1
kit 96 plate 1ml biospe pepfrac 600x600

BioSPE™ PepFrac – 96 wellplate & collection plates

96 Wellplate for peptide fractionation at basic pH - 1mL wells - Standard Capacity - 1/pk + Collection plate - 1mL - 9/pk + Triethylamine 0.1% (200mL)

Ref. : KT-Coll-96W-PepFrac.S8.1
kit 96 plate 1ml biospe pepfrac 600x600

BioSPE™ PepFrac – 96 wellplate & collection plates

96 Wellplate for peptide fractionation at basic pH - 1mL wells - High Capacity - 1/pk + Collection plate - 1mL - 9/pk + Triethylamine 0.1% (200mL)

Ref. : KT-Coll-96W-PepFrac.H8.1
kit tips 200ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 200µL & spin adapters

Kit of tips for peptide fractionation at basic pH - 200µL - High Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.H8.200.96
kit tips 200ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 200µL & spin adapters

Kit of tips for peptide fractionation at basic pH - 200µL - Standard Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.S8.200.96
kit tips 10ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 10µL & spin adapters

Kit of tips for peptide fractionation at basic pH - 10µL - Standard Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.S8.10.96
kit tips 10ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 10µL & spin adapters

Kit of tips for peptide fractionation at basic pH - 10µL - High Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.H8.10.96
kit tips 10ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 10µL Single Cell & spin adapters

Kit of tips for peptide fractionation at basic pH in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.SC8.10.96
kit tips 1ml biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 1mL & spin adapters

Kit of tips for peptide fractionation at basic pH - 1mL - Standard Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.S8.1000.96
kit tips 1ml biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 1mL & spin adapters

Kit of tips for peptide fractionation at basic pH - 1mL - High Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.H8.1000.96
kit spin micro biospe pepfrac 600x600

BioSPE™ PepFrac – µSpin

µSpin columns for peptide fractionation at basic pH - Standard Capacity - 50/pk + collection tubes - 450/pk + Triethylamine 0.1% (100mL)

Ref. : µSpin-PepFrac.S8.50
kit spin micro biospe pepfrac 600x600

BioSPE™ PepFrac – µSpin

µSpin columns for peptide fractionation at basic pH - High Capacity - 50/pk + collection tubes - 450/pk + Triethylamine 0.1% (100mL)

Ref. : µSpin-PepFrac.H8.50
kit spin mini biospe pepfrac 600x600

BioSPE™ PepFrac – Spin

Spin columns for peptide fractionation at basic pH - Standard Capacity - 50/pk + collection tubes - 450/pk + Triethylamine 0.1% (100mL)

Ref. : Spin-PepFrac.S8.50
kit spin mini biospe pepfrac 600x600

BioSPE™ PepFrac – Spin

Spin columns for peptide fractionation at basic pH - High Capacity - 50/pk + collection tubes - 450/pk + Triethylamine 0.1% (100mL)

Ref. : Spin-PepFrac.H8.50
kit spin 15ml biospe pepfrac 600x600

BioSPE™ PepFrac – Spin 15mL

Spin columns for peptide fractionation at basic pH - Standard Capacity - 15mL - 25/pk + collection tubes - 225/pk + Triethylamine 0.1% (100mL)

Ref. : Spin15-PepFrac.S8.25
kit spin 15ml biospe pepfrac 600x600

BioSPE™ PepFrac – Spin 15mL

Spin columns for peptide fractionation at basic pH - High Capacity - 15mL - 25/pk + collection tubes - 225/pk + Triethylamine 0.1% (100mL)

Ref. : Spin15-PepFrac.H8.25
kit spin 50ml biospe pepfrac 600x600

BioSPE™ PepFrac – Spin 50mL

Spin columns for peptide fractionation at basic pH - Standard Capacity - 50mL - 25/pk + collection tubes - 225/pk + Triethylamine 0.1% (100mL)

Ref. : Spin50-PepFrac.S8.25
kit spin 50ml biospe pepfrac 600x600

BioSPE™ PepFrac – Spin 50mL

Spin columns for peptide fractionation at basic pH - High Capacity - 50mL - 25/pk + collection tubes - 225/pk + Triethylamine 0.1% (100mL)

Ref. : Spin50-PepFrac.H8.25
cartridge biospe 1ml 600x600

BioSPE™ PepFrac – Cartridges 1mL

SPE cartridges for peptide fractionation at basic pH - Standard Capacity - 1mL - 50/pk + Triethylamine 0.1% (100mL)

Ref. : CAR1-PepFrac.S8.50
cartridge biospe 1ml 600x600

BioSPE™ PepFrac – Cartridges 1mL

SPE cartridges for peptide fractionation at basic pH - High Capacity - 1mL - 50/pk + Triethylamine 0.1% (100mL)

Ref. : CAR1-PepFrac.H8.50
cartridge biospe 3ml 600x600

BioSPE™ PepFrac – Cartridges 3mL

SPE cartridges for peptide fractionation at basic pH - Standard Capacity - 3mL - 50/pk + Triethylamine 0.1% (200mL)

Ref. : CAR3-PepFrac.S8.50
cartridge biospe 3ml 600x600

BioSPE™ PepFrac – Cartridges 3mL

SPE cartridges for peptide fractionation at basic pH - High Capacity - 3mL - 50/pk + Triethylamine 0.1% (200mL)

Ref. : CAR3-PepFrac.H8.50
cartridge biospe 6ml 600x600

BioSPE™ PepFrac – Cartridges 6mL

SPE cartridges for peptide fractionation at basic pH - Standard Capacity - 6mL - 25/pk + Triethylamine 0.1% (200mL)

Ref. : CAR6-PepFrac.S8.25
cartridge biospe 6ml 600x600

BioSPE™ PepFrac – Cartridges 6mL

SPE cartridges for peptide fractionation at basic pH - 6mL - 25/pk

Ref. : CAR6-PepFrac.H8.25