Desalinizzazione dei peptidi

Purificazione e desalinizzazione dei peptidi negli approcci proteomici bottom-up.

01 sf peptide desalting v2

BioSPE™ PurePep è utilizzato per un efficiente desaltazione dei peptidi dopo la digestione enzimatica, prima dell’analisi LC-MS/MS. Dopo il desalaggio del digest di HeLa su BioSPE™PurePep è stato identificato fino al 97% di proteine, con un buon recupero di peptidi sull’intero intervallo di polarità e una buona ripetibilità (RSD < 3%).

Per il desalaggio dei peptidi più idrofili, si consiglia l’uso di BioSPE™ PurePep Broad, in grado di trattenere efficacemente i piccoli peptidi polari. BioSPE™ PurePep Broad è stabile anche in condizioni di pH estreme (pH 0-14) e può asciugarsi durante il processo SPE senza conseguenze sui risultati.

I kit BioSPE™ PurePep e BioSPE™ PurePep Broad sono composti da:

  • Colonne di desalinizzazione
  • Adattatori (per la centrifugazione dei puntali) e/o strumenti di raccolta (provette da centrifuga o piastre di raccolta). Ogni kit contiene due strumenti di raccolta (uno per le fasi di condizionamento/caricamento/lavaggio e uno per la fase di eluizione)
  • Un protocollo dettagliato per una desalinizzazione facile e veloce

Per determinare il formato e la capacità più adatti alle proprie esigenze, consultare le tabelle nella scheda “Formati e capacità”.

ELENCO PRODOTTI

Nome del prodotto Prezzo del prodotto Product SKU Azioni del carrello
kit 96 plate 1ml collection plates x2 biospe

BioSPE™ PurePep – 96 wellplate & collection plates

96 Wellplate for peptide desalting - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-96W-PurePep.S.1
kit 96 plate 1ml collection plates x2 biospe

BioSPE™ PurePep – 96 wellplate & collection plates

96 Wellplate for peptide desalting - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-96W-PurePep.H.1
kit 96 plate 1ml collection plates x2 biospe

BioSPE™ PurePep Broad – 96 wellplate & collection plates

96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-96W-PurePep-Broad.H.1
kit 96 plate 1ml collection plates x2 biospe

BioSPE™ PurePep Broad – 96 wellplate & collection plates

96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-96W-PurePep-Broad.S.1
kit tips 10ul 600x600

BioSPE™ PurePep Broad – Tips 10µL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.S.10.96
kit tips 10ul 600x600

BioSPE™ PurePep Broad – Tips 10µL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.H.10.96
kit tips 10ul 600x600

BioSPE™ PurePep Broad – Tips 10µL Single Cell & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.SC.10.96
kit tips 1ml 600x600

BioSPE™ PurePep Broad – Tips 1mL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL -  Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.S.1000.96
kit tips 1ml 600x600

BioSPE™ PurePep Broad – Tips 1mL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.H.1000.96
kit tips 200ul 600x600

BioSPE™ PurePep Broad – Tips 200µL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.H.200.96
kit tips 200ul 600x600

BioSPE™ PurePep Broad – Tips 200µL & spin adapters

Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep-Broad.S.200.96
kit 96 plate microelution collection plates x2 biospe 600x600

BioSPE™ PurePep – 96 wellplate for microelution & collection plates

96 Wellplate for peptide desalting - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-µ96W-PurePep.H.1
kit 96 plate microelution collection plates x2 biospe 600x600

BioSPE™ PurePep – 96 wellplate for microelution & collection plates

96 Wellplate for peptide desalting - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-µ96W-PurePep.S.1
kit 96 plate microelution collection plates x2 biospe 600x600

BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates

96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-µ96W-PurePep-Broad.H.1
kit 96 plate microelution collection plates x2 biospe 600x600

BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates

96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk

Ref. : KT-Coll-µ96W-PurePep-Broad.S.1
kit tips 10ul 600x600

BioSPE™ PurePep – Tips 10µL & spin adapters

Kit of tips for peptide desalting - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.S.10.96
kit tips 10ul 600x600

BioSPE™ PurePep – Tips 10µL & spin adapters

Kit of tips for peptide desalting - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.H.10.96
kit tips 10ul 600x600

BioSPE™ PurePep – Tips 10µL Single Cell & spin adapters

Kit of tips for peptide desalting in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.SC.10.96
kit tips 1ml 600x600

BioSPE™ PurePep – Tips 1mL & spin adapters

Kit of tips for peptide desalting - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.S.1000.96
kit tips 1ml 600x600

BioSPE™ PurePep – Tips 1mL & spin adapters

Kit of tips for peptide desalting - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.H.1000.96
kit tips 200ul 600x600

BioSPE™ PurePep – Tips 200µL & spin adapters

Kit for peptide desalting containing: SPE Tips 200µL - Standard capacity - 96/pk | Spin adapters - 96/pk | 2mL collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.S.200.96
kit tips 200ul 600x600

BioSPE™ PurePep – Tips 200µL & spin adapters

Kit of tips for peptide desalting - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk

Ref. : KT-Adapt-Tips-PurePep.H.200.96
cartridge biospe 3ml 600x600

BioSPE™ PurePep Broad – Cartridges 3mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - High capacity - 50/pk

Ref. : CAR3-PurePep-Broad.H.50
kit spin mini 600x600

BioSPE™ PurePep – Spin

Spin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk

Ref. : Spin-PurePep.S.50
cartridge biospe 3ml 600x600

BioSPE™ PurePep Broad – Cartridges 3mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - Standard capacity - 50/pk

Ref. : CAR3-PurePep-Broad.S.50
cartridge biospe 6ml 600x600

BioSPE™ PurePep Broad – Cartridges 6mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - High capacity - 50/pk

Ref. : CAR6-PurePep-Broad.H.50
cartridge biospe 6ml 600x600

BioSPE™ PurePep Broad – Cartridges 6mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - Standard capacity - 50/pk

Ref. : CAR6-PurePep-Broad.S.50
kit spin mini 600x600

BioSPE™ PurePep Broad – Spin

Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk

Ref. : Spin-PurePep-Broad.S.50
kit spin mini 600x600

BioSPE™ PurePep Broad – Spin

Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk

Ref. : Spin-PurePep-Broad.H.50
kit spin 15ml 600x600

BioSPE™ PurePep Broad – Spin 15mL

Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 15mL - 25/pk + collection tubes - 50/pk

Ref. : Spin15-PurePep-Broad.S.25
kit spin 15ml 600x600

BioSPE™ PurePep Broad – Spin 15mL

Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 15mL - 25/pk + collection tubes - 50/pk

Ref. : Spin15-PurePep-Broad.H.25
kit spin 50ml 600x600

BioSPE™ PurePep Broad – Spin 50mL

Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50mL - 25/pk + collection tubes - 50/pk

Ref. : Spin50-PurePep-Broad.H.25
kit spin 50ml 600x600

BioSPE™ PurePep Broad – Spin 50mL

Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50mL - 25/pk + collection tubes - 50/pk

Ref. : Spin50-PurePep-Broad.S.25
kit spin micro 600x600

BioSPE™ PurePep Broad – µSpin

µSpin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk

Ref. : µSpin-PurePep-Broad.S.50
kit spin micro 600x600

BioSPE™ PurePep Broad – µSpin

µSpin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk

Ref. : µSpin-PurePep-Broad.H.50
kit spin micro 600x600

BioSPE™ PurePep – µSpin

µSpin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk

Ref. : µSpin-PurePep.S.50
cartridge biospe 1ml 600x600

BioSPE™ PurePep – Cartridges 1mL

SPE cartridges for peptide desalting - 1mL - Standard capacity - 50/pk

Ref. : CAR1-PurePep.S.50
cartridge biospe 1ml 600x600

BioSPE™ PurePep – Cartridges 1mL

SPE cartridges for peptide desalting - 1mL - High capacity - 50/pk

Ref. : CAR1-PurePep.H.50
cartridge biospe 3ml 600x600

BioSPE™ PurePep – Cartridges 3mL

SPE cartridges for peptide desalting - 3mL - Standard capacity - 50/pk

Ref. : CAR3-PurePep.S.50
cartridge biospe 3ml 600x600

BioSPE™ PurePep – Cartridges 3mL

SPE cartridges for peptide desalting - 3mL - High capacity - 50/pk

Ref. : CAR3-PurePep.H.50
cartridge biospe 6ml 600x600

BioSPE™ PurePep – Cartridges 6mL

SPE cartridges for peptide desalting - 6mL - Standard capacity - 50/pk

Ref. : CAR6-PurePep.S.50
cartridge biospe 6ml 600x600

BioSPE™ PurePep – Cartridges 6mL

SPE cartridges for peptide desalting - 6mL - High capacity - 50/pk

Ref. : CAR6-PurePep.H.50
kit spin mini 600x600

BioSPE™ PurePep – Spin

Spin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk

Ref. : Spin-PurePep.H.50
kit spin 15ml 600x600

BioSPE™ PurePep – Spin 15mL

Spin columns for peptide desalting - 15mL - High capacity - 25/pk + collection tubes - 50/pk

Ref. : Spin15-PurePep.H.25
kit spin 15ml 600x600

BioSPE™ PurePep – Spin 15mL

Spin columns for peptide desalting - 15mL - Standard capacity - 25/pk + collection tubes - 50/pk

Ref. : Spin15-PurePep.S.25
kit spin 50ml 600x600

BioSPE™ PurePep – Spin 50mL

Spin columns for peptide desalting - 50mL - High capacity - 25/pk + collection tubes - 50/pk

Ref. : Spin50-PurePep.H.25
kit spin 50ml 600x600

BioSPE™ PurePep – Spin 50mL

Spin columns for peptide desalting - 50mL - Standard capacity - 25/pk + collection tubes - 50/pk

Ref. : Spin50-PurePep.S.25
kit spin micro 600x600

BioSPE™ PurePep – µSpin

µSpin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk

Ref. : µSpin-PurePep.H.50
cartridge biospe 1ml 600x600

BioSPE™ PurePep Broad – Cartridges 1mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 50/pk

Ref. : CAR1-PurePep-Broad.H.50
cartridge biospe 1ml 600x600

BioSPE™ PurePep Broad – Cartridges 1mL

SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 50/pk

Ref. : CAR1-PurePep-Broad.S.50